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Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from the mouse kidney and HK-2 cells using a column-type animal tissue total RNA extraction and purification kit (Sangon Biotech Co., Ltd.), and then reverse transcribed to synthesize complementary DNA using a PrimeScript™ RT Reagent Kit with gDNA Eraser Kit (Takara Bio, Inc.)
كريم يعزز مرونة البشرة
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Nrf2 is also required for GCLM induction by 15d-PGJ 2 and physiological 4-HNE concentrations [84–85]
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