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At Δ Π = 220 mOsm, ER vesiculation rarely occurred, at Δ Π = 303 mOsm it did not reverse (Fig

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More specifically, the present invention provides materials and methods for improved sensitivity in the fluorescent (e.g., internally quenched fluorescence technology) detection of isopeptidases such as deubiquitinating enzymes and other ubiquitin-like protein deconjugases by use of diubiquitin protein substrates [91] and for measuring the isopeptidase activity of a deubiquitinase