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4 ROS reactive oxygen species CDNB 1-chloro-2,4-dinitrobenzene DTT dithiothreitol GCL γ-glutamate-cysteine ligase GSSGR glutathione reductase γ-GluCys γ-glutamylcysteine Ht hematocrit k GSHox unitary rate constant for the oxidation of GSH NAC N -acetylcysteine TFG total free glutathione ( i.e ., 2 × GSSG + GSH)