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Before blocking with 3% bovine serum albumin (BSA)-containing 0.1MPBS, antigen retrieval treatment with 0.01 M citrate buffer (pH6.0) at 95 °C for 40 min was performed

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Although conducted in a small patient population, preliminary results (Figure 1 and Table 4) suggest that opiorphin inhibits seminal NEP and may improve sperm motility parameters