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Fructose 1, 6-bisphosphatase (PFK1) binds to the transcription factor TEAD1 through a Hippo-independent pathway and forms a PFK1–TEAD1–YAP protein complex in glycolysis (Hardie et al., 2012), which, in turn, phosphorylates YAP at multiple sites (Mo et al., 2015)
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Various methods have been used to selectively enrich these proteins for proteomics, ranging from isolation of ‘white ghost’ membranes, to absorption of the highly abundant haemoglobin prior to analysis 16,17,18,19,20,21,22,23,24,25
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