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Primary antibodies: anti-GAPDH (Proteintech, Wuhan, China), anti-TGF-β1 (Proteintech, Wuhan, China), anti-Smad2/3 (Abcam, United Kingdom), anti-p-Smad2/3 (HUABIO, Hangzhou, China), anti-PI3K (Cell Signaling Technology, MA, United States), anti-p-AKT (Selleckchem, TX, United States), anti-p-mTOR (Proteintech, Wuhan, China), anti-p-ULK (ABclonal, Wuhan, China), anti-p-70S6K (ABclonal, Wuhan, China), anti-Beclin1 (ABclonal, Wuhan, China), anti-LC3 (Proteintech, Wuhan, China), anti-p62 (Proteintech, Wuhan, China), anti-BI1 (Santa Cruz Biotechnology, TX, United States), anti-Bax (Santa Cruz Biotechnology, TX, United States)。Secondary antibodies: goat anti-rabbit IgG (H + L)/HRP (Proteintech, Wuhan, China) for TGFβ1, Smad2/3, p-Smad2/3, PI3K, p-AKT, p-ULK, p-70S6K, Beclin1, LC3, p62 and GAPDH, while goat anti-mouse IgG (H + L)/HRP (Proteintech, Wuhan, China) for p-mTOR, BI1 and Bax

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Juweid, M