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Inefficient thermogenic mitochondrial respiration due to futile proton leak in a mouse model of fragile X syndrome

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Briefly, recombinant PDI protein (0.4 μM) was incubated with indicated compounds at 37 °C for 1 h in sodium phosphate buffer (100 mM sodium phosphate, 2 mM EDTA and 8 μM DTT, pH 7.0)