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The claudin-4 produced in the reaction solution was collected in the 100,000 g supernatant, adsorbed on Ni-Sepharose affinity resin, washed with wash buffer A [50 mM Tris-HCl buffer (pH 7.0), containing 0.05% βDDM, 0.002% CHS, 20 mM imidazole and 400 mM NaCl] and then eluted with 500 mM imidazole in the same buffer

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However, exposure to polystyrene cups and containers generally won’t be severe enough to cause acute symptoms