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The ATCC supplied the human epithelial hepatocellular carcinoma (HepG2) cells, which were incubated at 37 °C and 5% CO 2 in DMEM-Adv supplemented with 10% FBS, 2 mM L-glutamine, and 1% penicillin–streptomycin [31]

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Superoxide is produced by a variety of enzyme systems, and mitochondria appear to be an important source of myocardial ROS in the failing heart [136]