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Transcriptome profiling of human post-mortem tissues (obtained from people aged between 16 and 102 years) found prominent and complex changes in gene expression in oligodendrocytes and astrocytes, whereas the neuronal transcriptome remained more or less undisturbed

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2.1.3.2 Advanced precision modification: bio-orthogonal click chemistry and site-specific ligation Tyrosine-targeted alkyne-azide cycloaddition (CuAAC): tyrosine residues can be selectively modified via diazonium chemistry (pH 9, 4 °C) to introduce terminal alkynes, which then undergo copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) to generate 1,4-disubstituted 1,2,3-triazole linkages (Figure 2E