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A small controlled human trial conducted by Leyden and colleagues for Procyte Corporation in the early 1990s reported statistically significant increases in follicle size in GHK-Cu-treated scalp regions compared to vehicle control
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Cells were collected after 72 h and lysed by gentle nutation in wash buffer (50 mM HEPES, 150 mM NaCl, 2.5 mM MgCl 2 , 1 mM TCEP, pH 7.4) supplemented with 0.4% CHAPS and Protease Inhibitor (Roche) for 1 h
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