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Following granule exocytosis, perforin facilitates the delivery of granzymes into the cytosol of the target cell where they cleave numerous substrates, including caspases, resulting in the rapid induction of apoptosis 7,8

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Real-Time Quantitative Reverse Transcription PCR (qRT‒PCR) Analysis Total RNA from skin tissues was isolated using TRIzol reagent (Sigma, Milwaukee, WI, USA), and 1 μg of total RNA was reverse-transcribed into cDNA