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The needle should enter the joint without significant resistance
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SIZE:70ml
5 µl of metabolites suspension was loaded and resolved on an Acquity UPLC BEH C18 column (1.7 μm, 2.1 × 100 mm, Waters) using mobile phases of water with 0.1% formic acid (buffer A) and acetonitrile with 0.1% formic acid (buffer B) with a flow rate of 0.3 ml/min and 8 minutes long gradient
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