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While BCL-2 protein expression upon CB-839 treatment remained stable even at maximal concentrations of 100 μM, MCL-1 protein levels increased in both cell lines in cells incubated with CB-839 compared with control cells, indicating that the HG-3 and MEC-1 cells had an MCL-1 protein dependency in response to apoptotic stimuli ( Supplemental Figure 3 )
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