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2.1.3.2 Advanced precision modification: bio-orthogonal click chemistry and site-specific ligation Tyrosine-targeted alkyne-azide cycloaddition (CuAAC): tyrosine residues can be selectively modified via diazonium chemistry (pH 9, 4 °C) to introduce terminal alkynes, which then undergo copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) to generate 1,4-disubstituted 1,2,3-triazole linkages (Figure 2E