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Additionally, the receptor to which a polypeptide of the invention binds can be identified by numerous methods known to those of skill in the art, for example, ligand panning and FACS sorting (Coligan, et al., Current Protocols in Immun., 1(2),Chapter5, (1991))

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Advanced liver disease can alter the levels of B12 binding proteins in the blood, making total serum B12 measurements unreliable—levels may appear normal or even elevated despite functional deficiency