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The PCR reaction consisted of an initial denaturation step at 98°C for 2 min, followed by 35 cycles of 10 s at 98°C, 30 s at a primer specific annealing temperature (Supplementary Table 1), and 30 s per 1 kb of predicted PCR product at 72°C, and a final extension step (10 min at 72°C)
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10.1039/D3DT01517C [DOI] [PubMed] [Google Scholar] 268.Deveci, H
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