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In some embodiments, preparation of certain cations of Formulas I and IA may occur by alkylating the N-positions of the quinoline scaffold using, for example, iodomethane or methyl trifluoromethanesulfonate (see Scheme 1)

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Hence, knowing which proteins latently interact with CRBN–MGD E3 ligase complexes, even if not yet degraded, will inform CRBN target discovery