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The chosen concentration was also consistent with those reported in previous studies investigating transplacental transport, which used similar concentrations, and even exceeded, using up to 1,000 ฮผg/mL of PS-MP in cell lines ( The observed increase in cytotoxicity confirms the findings from several studies demonstrating PS-MNP cytotoxicity in pregnant mice and their offspring (73, 74), placental cell lines (75, 76)
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A scRNA-seq analysis of brain PVMs in germ-free (GF) and specific pathogen-free (SPF) mice revealed specific gene signatures associated with the presence or absence of microbiota [44]
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