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fumigatus genome (98) has accelerated gene structure and function studies and made possible comparative genomic analyses with other sequenced Aspergillus species ( Aspergillus oryzae and Aspergillus nidulans ), as well as other genera of pathogenic (e.g., Candida albicans and Cryptococcus neoformans ) and nonpathogenic (e.g., Saccharomyces cerevisiae ) fungi
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Beads were then washed twice with 5 ml of equilibration buffer and twice with 5 ml of capture-wash buffer (18 mM HEPES pH 7.4, 2 M NaCl, 1 mM MgCl 2 , 10% (v/v) glycerol, 0.003% (v/v) IGEPAL CA-630, 0.033 mg ml โ1 recombinant albumin and 0.005 mg ml โ1 yeast tRNA), then incubated with 1 nmol 3โฒ-biotinylated DNA competitor oligonucleotide (IDT) complementary to the capture oligonucleotide (Supplementary Table 4) in 18 mM HEPES pH 7.4, 1 M NaCl, 1 mM MgCl 2 , 10% (v/v) glycerol, 0.003% (v/v) IGEPAL CA-630, 0.033 mg ml โ1 recombinant albumin and 0.005 mg ml โ1 yeast tRNA, at 25 ยฐC for 2 h with end-over-end rotation
