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Irisin treatment significantly decreased the Bax/Bcl-2 ratio, while NAM treatment markedly increased it (Fig

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DPD and SHMT levels were determined using ELISA kits (MyBioSource, CA, United States), and cysteine/methionine/reduced glutathione levels were measured using assay kits (Sigma-Aldrich, MO, United States or Cell Biolabs, CA, United States)