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While acetaminophen alone is not effective for severe pain, it is combined with nonsteroidal anti-inflammatory drugs (NSAIDs) to provide stronger relief without the risks associated with opioid-based medications

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Detection wavelengths were set at 220 nm for ITC-Cys and ITC-Cyclic-Cys conjugates, 280 nm for ITC-CysGly conjugates, and 240 nm for ITC-Lys conjugate