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and the reduction of leukocyte adhesion ( Betaine is a methyl derivative of glycine that is primarily obtained from dietary sources but is also endogenously synthesized in the liver from choline ( Betaine is stored in the kidney and liver, and serves three important physiological functions: it acts as an osmolyte that regulates cell volume, it acts as a chemical chaperone that protects against protein degradation, and it serves as a methyl donor in the methionine cycle which detoxifies homocysteine to methionineβa process that is crucial for numerous cellular functions ( Although homocysteine is commonly associated with cardiovascular diseases, there is increasing evidence supporting its strong correlation with CKD and renal injury ( in vitro evidence suggests that its antioxidant effects are mediated through the upregulation of endogenous non-enzymatic antioxidant defense systems and through the formation of physical protective barriers around cells ( Flaxseed, which is rich in alpha-linoleic acids, fiber, flavonoids, and lignans, is a widely used dietary supplement ( Taken together, our findings suggest that combining GA, melatonin, and betaine may offer a novel complementary therapeutic approach for attenuating the pathophysiological features of diabetes and CKD
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Methods Computer-aided molecular design, synthesis, and characterization All the computer-aided molecular design studies were performed in SZMAP/GamePlan 37 and vBrood 38 modules in OpenEye software and Molecular Operating Environment, MOE 39
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