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The catheters were exteriorized through the dorsal skin and flushed with heparinized saline before and after blood sample collection and filled with heparin-glycerol locking solution (6 mL glycerol, 3 mL saline, 0.5 mL gentamycin (l OOmg/mL), 0.5 mL heparin (10,000 u/mL)) when not sampled for more than 8 hours