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Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) characterizes stepwise bile acid perturbations across DKD stages (199), demonstrating research value but constrained routine applicability given costs exceeding $800 per analysis

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Discussion The Aldh2*2 KI mouse strain generated in this study has a single nucleotide substitution that is homologous to the rs671 substitution of human ALDH2*2 allele

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