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Lysates were diluted with a pulldown buffer (20 mM Tris-HCl pH 7.5, 1% Triton X-100, 100 mM NaCl, supplemented with protease and phosphatase inhibitors) and incubated overnight with Dynabeads M-270 Streptavidin at 4 °C on a rotator according to the manufacturer’s instructions
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Published 2018 May 24
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