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While cytosol, peroxisomes, mitochondria, and plastids in Arabidopsis wild-type plants maintain a highly reduced glutathione buffer with redox potentials of less than −310 mV, the ER is far less reducing with E GSH values of less than −240 mV (Meyer et al., E GSH with high specificity due to selective mediation of thiol/disulfide exchange reaction between glutathione and roGFP by GRXs (Meyer et al., E GSH is outside the measuring range of these probes thus limiting their usability

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Despite the fact that the main function of the MTHFR enzyme is to regulate the availability of 5-MTHF for homocysteine remethylation, the pathological consequences of functional variants of MTHFR gene cannot only be attributed to the increase in homocysteine levels