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Sections were rinsed with 80% ethanol followed by water, differentiated in 0.05% Li2CO3 (Sigma-Aldrich #255823) solution for 30 min, rinsed in 70% ethanol and water, then counterstained with cresyl violet (1 mg/mL cresyl violet and 0.05 mg/mL oxalic acid), and rinsed successively in water, 70% ethanol, 95% ethanol, 100% ethanol and xylene