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2.13 Measurement of intracellular ROS levels Intracellular ROS was measured using 2′,7′-Dichlorodihydrofluorescein diacetate (H 2 DCF-DA) probe by following the protocol described in a previous study ( 2 DCF-DA (final concentration, 25 μM in M9) was added to each well and the samples were incubated at room temperature for 1 h