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An initial denaturation was carried out for 45 s at 94 ° C, 24 cycles of denaturation for 15 s at 94 ° C, annealing-extension at 68 ° C for 12 min, and a final extension at 72 ° C for 10 min using the following primers: 5′-CACTATCAACATATTCATCTCTTGTTTC-3′ (forward) and 5′-GCTCTATGGACACCAATAGTTCCAAGGG-3′ (reverse)
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Colder temperatures are recommended for optimal stability
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